Malaria is a public health problem in some parts of Bangladesh, particularly in 13 districts in the north-east and south-east areas. Among them Bandarban district is highly endemic. There is a need for updating preventive measures on malaria for suitable control strategies among tribal populations. On the basis of this need the present study assessed the perception and utilization of preventive measures of malaria among Tribal adults in higher endemic community. A community based cross-sectional study was done among 316 Tribals. There were 63.61% female and 36.39% male respondents. One third of the respondent’s levels of education were no schooling and 51.6% were housewives. Most of the respondents lived in nuclear family. All of the respondents had heard about malaria. About 98.1% of the respondents correctly knew that malaria is transmitted by mosquito bite and fever was the recognized symptom for malaria (90.5%). Most of the respondents 94.0% knew about malaria preventive measures. The most commonly used preventive measures were Long Lasting Insecticide Treated Net (LLIN) followed by clean surroundings. More than half 56.3% of the respondents did not know about mosquito biting time. More than one third 44.0% of the respondents used LLIN after 10pm and it is associated with sociodemographic characteristics (p=<0.05). Only 20% respondents ever had malaria, among them 55.4% respondent’s malaria was diagnosed more than 3 days and 93.8% started treatment within 1 day after diagnosis of malaria. Utilization of preventive measures is associated with sociodemographic characteristics (p=<0.05). After multivariate analysis, no. of family member are associated with all family member at home sleep under LLIN (p=0.003, OR=0.323, 95% CI for OR from 0.152 to 0.687) while controlling others variables. Awareness should be increased regarding correct utilization of preventive measures of malaria in all community people who lived in malaria endemic areas.