Poster Presentation First Malaria World Congress 2018

Vesicular BioID reveals transmission-blocking vaccine candidates (#476)

Jessica Kehrer 1 , Dominik Ricken 1 , Leanne Strauss 1 , Emma Pietsch 1 , Danny Balitissen 1 , Friedrich Frischknecht 1
  1. University of Heidelberg, Germany, Heidelberg, BADEN-W├╝RTTEMBERG, Germany

Life cycle progression of the malaria parasite Plasmodiumis dependent on transmission stages such as gametocytes,ookinetes and sporozoites. Gametocyte egress from the red blood cell and motility of ookinetes and sporozoites relyon the secretion of proteins from specialized secretoryvesicles such as osmiophilic bodies and micronemes. In order to identify hitherto unknown proteins with a potential to be important during parasite transmission we have defined the content of these vesicles in the rodent model organism Plasmodium bergheiusing proximity dependent biotinylation (BioID) and mass spectrometry. Furthermore we identified the sporozoite secretome by mass spectrometry. We now use gene deletion of selected candidates and have identified important functions of essential proteins involved in transmission. Our data sets the basis for a better understanding of parasite egress, motility and transmission.